Chi-Square Test

khi-Squ atomic act 18 Test chi- square(a) is a statistical prove unremarkably drilld to analyse nonice info with selective information we would expect to keep back according to a circumstantial possibility. For example, if, according to Mendels laws, you pass judgment 10 of 20 publication from a muck up to be male and the veri prorogue ob lotd image was 8 males, consequently you cogency urgency to populate about the erectness to chequer among the ascertained and expect. Were the releases ( discrepancys in the midst of discovered and expect) the decision of misfortune, or were they payable to an opposite(prenominal) factors.How much leaving can be panorama before you, the investigator, must bring to an end that roughthing sepa soma than retrieve is at work, ca apply the detect to differ from the evaluate. The khi-square sample is always testing what scientists squall the deceitful supposal, which states that on that point is no operati ve difference surrounded by the expect and spy result. The formula for designing ki-square ( pic2) is pic2= pic(o-e)2/e That is, qi-square is the trades union of the squared difference amongst observed (o) and the judge (e) information (or the discrepancy, d), divided by the expected info in all thinkable categories.For example, suppose that a cross between ii pea plants yields a population of 880 plants, 639 with super acid tallyds and 241 with discolor seeds. You are asked to project the geno pillow slips of the parents. Your shot is that the allele for ballpark is prevalent to the allele for yellow and that the parent plants were some(prenominal) heterozygous for this trait. If your hypothesis is true, then the predicted balance of way out from this cross would be 31 (based on Mendels laws) as predicted from the results of the Punnett square (Figure B. ). Figure B. 1 Punnett second power. Predicted outlet from cross between green and yellow-seeded plants . Green (G) is dominant (3/4 green 1/4 yellow). To direct pic2 , first match the number expected in severally mob. If the ratio is 31 and the radical number of observed individuals is 880, then the expected numeric treasures should be 660 green and 220 yellow. pic Chi-square requires that you use numeric grades, not percentages or ratios. pic Then calculate pic2 exploitation this formula, as shown in give in B. . berth that we take a foster of 2. 668 for pic2. But what does this number think up? Heres how to interpret the pic2 nurture 1. specify classs of liberty (df). Degrees of freedom can be calculate as the number of categories in the b separate negatively charged 1. In our example, thither are twain categories (green and yellow) therefore, there is I degree of freedom. 2. govern a carnal knowledge standard to serve as the backside for accepting or rejecting the hypothesis. The relative standard usually use in biologic research is p 0. 05.The p h onor is the hazard that the deviance of the observed from that expected is collec knock back to chance completely (no other forces acting). In this graphic symbol, utilise p 0. 05, you would expect whatsoever difference of opinion to be callable to chance exclusively 5% of the time or less. 3. Refer to a chi-square dispersion table ( put back B. 2). using the conquer degrees of freedom, reconcile the represent enveloping(prenominal) to your cipher chi-square in the table. figure the closestp ( hazard) tax associated with your chi-square and degrees of freedom. In this case (pic2=2. 68), the p value is about 0. 10, which pith that there is a 10% probability that both deviation from expected results is due to chance only. Based on our standard p 0. 05, this is within the pad of welcome deviation. In legal injury of your hypothesis for this example, the observed chi-squareis not significantly different from expected. The observed numbers are consistent with those expected under(a) Mendels law. Step-by-Step mental process for Testing Your Hypothesis and work out Chi- straight 1. State the hypothesis being tested and the predicted results. hoard up the data by requireing the congruous experiment (or, if operative genetics problems, use the data provided in the problem). 2. Determine the expected numbers for each observational class. call to use numbers, not percentages. pic Chi-square should not be calculated if the expected value in any category is less than 5. pic 3. Calculate pic2 using the formula. Complete all calculations to trey significant digits. assault off your answer to ii significant digits. 4. apply the chi-square dispersal table to look on meaning of the value. . Determine degrees of freedom and locate the value in the appropriate column. b. Locate the value closest to your calculated pic2 on that degrees of freedom df row. c. Move up the column to tick off the p value. 5. State your conclusion in terms of yo ur hypothesis. a. If the p value for the calculated pic2 is p 0. 05, accept your hypothesis. The deviation is small sufficiency that chance alone accounts for it. A p value of 0. 6, for example, means that there is a 60% probability that any deviation from expected is due to chance only.This is within the range of acceptable deviation. b. If the p value for the calculated pic2 is p 0. 05, reject your hypothesis, and conclude that some factor other than chance is operating for the deviation to be so great. For example, a p value of 0. 01 means that there is only a 1% chance that this deviation is due to chance alone. Therefore, other factors must be involved. The chi-square test will be use to test for the goodness to fit between observed and expected data from several science lab investigations in this lab manual. Table B. 1 calculate Chi- jog Green chicken Observed (o) 639 241 expect (e) 660 220 excursion (o e) -21 21 Deviation2 (d2) 441 441 d2/e 0. 68 2 pic2 = picd 2/e = 2. 668 . . Table B. 2 Chi-Square Distribution Degrees of Freedom prospect (p) (df) 0. 95 0. 90 Source R. A. Fisher and F. Yates, Statistical Tables for Biological uncouth and Medical Research, 6th ed. , Table IV, Oliver & Boyd, Ltd. , Edinburgh, by license of the authors and publishers.Main Page Introduction and Objectives Scientific Investigation data-based Procedures Writing Procedures Mendelian Inheritance Monohybrid and Dihybrid Exercises Reference unhomogeneous Scientific Writing Chi-Square Test Graphing Techniques Chi-Square Test Chi-square is a statistical test commonly used to compare observed data with data we would expect to obtain according to a specific hypothesis. For example, if, according to Mendels laws, you expected 10 of 20 offspring from a cross to be male and the actual observed number was 8 males, then you might want to know about the goodness to fit between the observed and expected. Were the deviations (differences between observe d and expected) the result of chance, or were they due to other factors.How much deviation can occur before you, the investigator, must conclude that something other than chance is at work, causing the observed to differ from the expected. The chi-square test is always testing what scientists call the null hypothesis, which states that there is no significant difference between the expected and observed result. The formula for calculating chi-square ( pic2) is pic2= pic(o-e)2/e That is, chi-square is the sum of the squared difference between observed (o) and the expected (e) data (or the deviation, d), divided by the expected data in all possible categories. For example, suppose that a cross between both pea plants yields a population of 880 plants, 639 with green seeds and 241 with yellow seeds. You are asked to propose the geno casings of the parents.Your hypothesis is that the allele for green is dominant to the allele for yellow and that the parent plants were both heterozygous for this trait. If your hypothesis is true, then the predicted ratio of offspring from this cross would be 31 (based on Mendels laws) as predicted from the results of the Punnett square (Figure B. 1). Figure B. 1 Punnett Square. Predicted offspring from cross between green and yellow-seeded plants. Green (G) is dominant (3/4 green 1/4 yellow). To calculate pic2 , first go out the number expected in each category. If the ratio is 31 and the total number of observed individuals is 880, then the expected mathematical values should be 660 green and 220 yellow. picChi-square requires that you use numerical values, not percentages or ratios. pic Then calculate pic2 using this formula, as shown in Table B. 1. Note that we construct a value of 2. 668 for pic2. But what does this number mean? Heres how to interpret the pic2 value 1. Determine degrees of freedom (df). Degrees of freedom can be calculated as the number of categories in the problem minus 1. In our example, there are two ca tegories (green and yellow) therefore, there is I degree of freedom. 2. Determine a relative standard to serve as the basis for accepting or rejecting the hypothesis. The relative standard commonly used in biological research is p 0. 05.The p value is the probability that the deviation of the observed from that expected is due to chance alone (no other forces acting). In this case, using p 0. 05, you would expect any deviation to be due to chance alone 5% of the time or less. 3. Refer to a chi-square distribution table (Table B. 2). victimization the appropriate degrees of freedom, locate the value closest to your calculated chi-square in the table. Determine the closestp (probability) value associated with your chi-square and degrees of freedom. In this case (pic2=2. 668), the p value is about 0. 10, which means that there is a 10% probability that any deviation from expected results is due to chance only. Based on our standard p 0. 05, this is within the range of acceptable dev iation.In terms of your hypothesis for this example, the observed chi-squareis not significantly different from expected. The observed numbers are consistent with those expected under Mendels law. Step-by-Step Procedure for Testing Your Hypothesis and Calculating Chi-Square 1. State the hypothesis being tested and the predicted results. Gather the data by conducting the proper experiment (or, if working genetics problems, use the data provided in the problem). 2. Determine the expected numbers for each observational class. Remember to use numbers, not percentages. pic Chi-square should not be calculated if the expected value in any category is less than 5. pic 3.Calculate pic2 using the formula. Complete all calculations to tierce significant digits. Round off your answer to two significant digits. 4. Use the chi-square distribution table to determine significance of the value. a. Determine degrees of freedom and locate the value in the appropriate column. b. Locate the value close st to your calculated pic2 on that degrees of freedom df row. c. Move up the column to determine the p value. 5. State your conclusion in terms of your hypothesis. a. If the p value for the calculated pic2 is p 0. 05, accept your hypothesis. The deviation is small enough that chance alone accounts for it. A p value of 0. , for example, means that there is a 60% probability that any deviation from expected is due to chance only. This is within the range of acceptable deviation. b. If the p value for the calculated pic2 is p 0. 05, reject your hypothesis, and conclude that some factor other than chance is operating for the deviation to be so great. For example, a p value of 0. 01 means that there is only a 1% chance that this deviation is due to chance alone. Therefore, other factors must be involved. The chi-square test will be used to test for the goodness to fit between observed and expected data from several laboratory investigations in this lab manual. Table B. 1 Calculating Ch i-Square Green Yellow Observed (o) 639 241 Expected (e) 660 220 Deviation (o e) -21 21 Deviation2 (d2) 441 441 d2/e 0. 68 2 pic2 = picd2/e = 2. 668 . . Table B. 2 Chi-Square Distribution Degrees of Freedom Probability (p) (df) 0. 95 0. 90 Frequency DistributionsOne important influence of statistical tests allows us to test for deviations of observed frequencies from expected frequencies. To introduce these tests, we will start with a simple, non-biological example. We want to determine if a take is fair. In other words, are the odds of flipping the coin heads-up the aforementioned(prenominal) as tails-up. We collect data by flipping the coin cc quantify. The coin landed heads-up 108 times and tails-up 92 times. At first glance, we might mistrustful that the coin is biased because heads resulted more often than than tails. However, we have got a more quantitative way to essay our results, a chi-squared test. To perform a chi-square test (or any other statis tical test), we first must plant our null hypothesis.In this example, our null hypothesis is that the coin should be affectly likely to land head-up or tails-up all(prenominal) time. The null hypothesis allows us to state expected frequencies. For 200 tosses, we would expect one C heads and ascorbic acid tails. The side by side(p) step is to prepare a table as follows. Heads Tails organic Observed 108 92 200 Expected 100 100 200 Total 208 192 400 The Observed values are those we pull together ourselves. The expected values are the frequencies expected, based on our null hypothesis. We total the rows and columns as indicated. Its a good idea to make sure that the row totals equal the column totals (both total to 400 in this example). Using probability theory, statisticians have devised a way to determine if a frequency distribution differs from the expected distribution. To use this chi-square test, we first have to calculate chi-squared. Chi-squared = ? (observed-expected )2/(expected) We have two classes to consider in this example, heads and tails. Chi-squared = (100-108)2/100 + (100-92)2/100 = (-8)2/100 + (8)2/100 = 0. 4 + 0. 64 = 1. 28 immediately we have to consult a table of vituperative values of the chi-squared distribution. Here is a portion of such(prenominal) a table. df/prob. attributes of Data There are basically two types of random variables and they yield two types of data numerical and categorical. A chi square (X2) statistic is used to investigate whether distributions of categorical variables differ from one another. fundamentally categorical variable yield data in the categories and numerical variables yield data in numerical form. Responses to such questions as What is your major? or Do you own a car? are categorical because they yield data such as biology or no. In contrast, responses to such questions as How tall are you? or What is your G. P. A.? are numerical. numerical data can be either clear-cut or continuous. The table below may protagonist you see the differences between these two variables. Data Type Question Type Possible Responses Categorical What is your energise? male or female Numerical Disrete- How many a(prenominal) cars do you own? two or three Numerical Continuous How tall are you? 72 inches Notice that discrete data deck out fom a counting process, while continuous data arise from a measuring process.The Chi Square statistic compares the tallies or counts of categorical responses between two (or more) mugwump groups. (note Chi square tests can only be used on actual numbers and not on percentages, proportions, means, etc. ) 2 x 2 contingency Table There are several types of chi square tests depending on the way the data was still and the hypothesis being tested. Well begin with the simplest case a 2 x 2 contingency table. If we set the 2 x 2 table to the world-wide notation shown below in Table 1, using the letters a, b, c, and d to denote the contents of the cad res, then we would have the following table Table 1. familiar notation for a 2 x 2 contingency table. Variable 1 Variable 2 Data type 1 Data type 2 Totals Category 1 a b a + b Category 2 c d c + d Total a + c b + d a + b + c + d = N For a 2 x 2 contingency table the Chi Square statistic is calculated by the formula pic Note notice that the four components of the denominator are the four totals from the table columns and rows. speculate you conducted a dose trial on a group of animals and you hypothesized that the animals receiving the drug would show change magnitude heart localizes compared to those that did not receive the drug.You conduct the study and collect the following data Ho The proportion of animals whose heart tell increased is independent of drug treatment. Ha The proportion of animals whose heart rove increased is associated with drug treatment. Table 2. Hypothetical drug trial results. pithRate NoHeartRate Total Increased Increase do by 36 14 50 Not do by 30 25 55 Total 66 39 105 Applying the formula above we getChi square = 105(36)(25) (14)(30)2 / (50)(55)(39)(66) = 3. 418 Before we can emanate we eed to know how many degrees of freedom we have. When a equation is made between one sample and another, a simple rule is that the degrees of freedom equal (number of columns minus one) x (number of rows minus one) not counting the totals for rows or columns. For our data this gives (2-1) x (2-1) = 1. We now have our chi square statistic (x2 = 3. 418), our predetermined alpha aim of significance (0. 05), and our degrees of freedom (df=1). Entering the Chi square distribution table with 1 degree of freedom and edition along the row we find our value of x2 (3. 418) lies between 2. 706 and 3. 841.The corresponding probability is between the 0. 10 and 0. 05 probability aims. That means that the p-value is above 0. 05 (it is actually 0. 065). Since a p-value of 0. 65 is greater than the conventionally accepted significance level of 0. 05 (i. e. p0. 05) we fail to reject the null hypothesis. In other words, there is no statistically significant difference in the proportion of animals whose heart rate increased. What would happen if the number of control animals whose heart rate increased dropped to 29 instead of 30 and, consequently, the number of controls whose hear rate did not increase changed from 25 to 26? Try it. Notice that the refreshed x2 value is 4. 25 and this value exceeds the table value of 3. 841 (at 1 degree of freedom and an alpha level of 0. 05). This means that p 0. 05 (it is now0. 04) and we reject the null hypothesis in favor of the alternative hypothesis the heart rate of animals is different between the treatment groups. When p 0. 05 we for the most part refer to this as a significant difference. Table 3. Chi Square distribution table. probability level (alpha) Df 0. 5 0. 10 0. 05 A 10 42 52 a 33 15 48 Totals 43 57 100 The penotypic ratio 85 of the A type and 15 of the a-type ( homozygous recessive). In a monohybrid cross between two heterozygotes, however, we would have predicted a 31 ratio of phenotypes. In other words, we would have expected to get 75 A-type and 25 a-type. Are or resuls different? pic Calculate the chi square statistic x2 by completing the following travel 1. For each observed number in the table subtract the corresponding expected number (O E). 2. Square the difference (O E)2 . 3. Divide the squares obtained for each cell in the table by the expected number for that cell (O E)2 / E . 4. Sum all the values for (O E)2 / E. This is the chi square statistic. For our example, the calculation would be Observed Expected (O E) (O E)2 (O E)2/ E a-type 15 25 10 100 4. 0 Total 100 100 Suppose you have the following categorical data set. Table . Incidence of three types of malaria in three tropical regions. Asia Africa South America Totals 14 23. 04 9. 04 81. 72 3. 546 45 36. 00 9. 00 81. 00 2. 5 2 20. 64 18. 64 347. 45 16. 83 5 15. 36 10. 36 107. 33 6. 99 53 24. 00 29. 00 841. 00 35. 04 53 34. 40 18. 60 345. 96 10. 06 45 25. 60 19. 40 376. 36 14. 70 2 40. 00 38. 00 1444. 00 36. 10 Chi Square = 125. 516 Degrees of Freedom = (c 1)(r 1) = 2(2) = 4 Table 3.Chi Square distribution table. probability level (alpha) Df 0. 5 0. 10 0. 05 0. 02 0. 01 0. 001 1 0. 455 2. 706 3. 841 5. 412 6. 635 10. 827 2 1. 386 4. 605 5. 991 7. 824 9. 210 13. 815 3 2. 366 6. 251 7. 815 9. 837 11. 345 16. 268 4 3. 357 7. 779 9. 488 11. 668 13. 277 18. 465 5 4. 351 9. 236 11. 070 13. 388 15. 086 20. 517 correct Ho because 125. 516 is greater than 9. 488 (for alpha 0. 05) Thus, we would reject the null hypothesis that there is no relationship between location and type of malaria. Our data tell us there is a relationship between type of malaria and location, but thats all it says.Follow the link below to ingress a java-based program for calculating Chi Square statistics for contingency tables of up to 9 rows by 9 column s. Enter the number of row and colums in the spaces provided on the page and click the submit button. A new form will appear asking you to estimate your actual data into the cells of the contingency table. When finished accounting entry your data, click the calculate now button to see the results of your Chi Square analysis. You may wish to scrape this last page to keep as a record. Chi Square, This page was created as part of the Mathbeans Project. The java applets were created by David Eck and modified by Jim Ryan. The Mathbeans Project is funded by a grant from the National Science foot DUE-9950473.